alpha-mem medium Search Results


90
EuroClone α-minimum essential medium/endothelial cell basal medium-1 (α-mem/ebm) (3:1)
α Minimum Essential Medium/Endothelial Cell Basal Medium 1 (α Mem/Ebm) (3:1), supplied by EuroClone, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ScienCell modified eagles medium (mem)
Dicarbonyl stress in human periodontal ligament cells in high glucose concentration cultures in vitro. (A) Protein glycation by methylglyoxal (MG) forming arginine-derived MG-H1 adduct. (B) Metabolism of MG by the glyoxalase system. (C–I) Culture of periodontal ligament <t>fibroblasts</t> <t>(PDLFs)</t> for 3 days and effect of glucose concentration. (C) Viable cell number and effect of initial glucose concentration. Key: x─ ─ x, 5 mM glucose; ○─○, 8 mM glucose and ●─●, 25 mM glucose. (D) Cellular MG content (n=4). (E) MG concentration of the culture <t>medium</t> (n=4). (F) MG-H1 content of cell protein. (G) Flux of release of MG-H1 free adduct into the culture medium. (H) FL content of cell protein. (I) Flux of release of FL free adduct into the culture medium. Data are mean±SD (C) n=3; (D–G) n=4; (H and I) n=5). *P<0.05; **p<0.01; ***p<0.001; Student’s t-test . FL, N ε -fructosyl-lysine; HG, high glucose; LG, low glucose.
Modified Eagles Medium (Mem), supplied by ScienCell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Biowest SAS alpha-minimum essential medium
Dicarbonyl stress in human periodontal ligament cells in high glucose concentration cultures in vitro. (A) Protein glycation by methylglyoxal (MG) forming arginine-derived MG-H1 adduct. (B) Metabolism of MG by the glyoxalase system. (C–I) Culture of periodontal ligament <t>fibroblasts</t> <t>(PDLFs)</t> for 3 days and effect of glucose concentration. (C) Viable cell number and effect of initial glucose concentration. Key: x─ ─ x, 5 mM glucose; ○─○, 8 mM glucose and ●─●, 25 mM glucose. (D) Cellular MG content (n=4). (E) MG concentration of the culture <t>medium</t> (n=4). (F) MG-H1 content of cell protein. (G) Flux of release of MG-H1 free adduct into the culture medium. (H) FL content of cell protein. (I) Flux of release of FL free adduct into the culture medium. Data are mean±SD (C) n=3; (D–G) n=4; (H and I) n=5). *P<0.05; **p<0.01; ***p<0.001; Student’s t-test . FL, N ε -fructosyl-lysine; HG, high glucose; LG, low glucose.
Alpha Minimum Essential Medium, supplied by Biowest SAS, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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BioWhittaker Molecular Applications alpha modified eagle’s minimum essential medium
Dicarbonyl stress in human periodontal ligament cells in high glucose concentration cultures in vitro. (A) Protein glycation by methylglyoxal (MG) forming arginine-derived MG-H1 adduct. (B) Metabolism of MG by the glyoxalase system. (C–I) Culture of periodontal ligament <t>fibroblasts</t> <t>(PDLFs)</t> for 3 days and effect of glucose concentration. (C) Viable cell number and effect of initial glucose concentration. Key: x─ ─ x, 5 mM glucose; ○─○, 8 mM glucose and ●─●, 25 mM glucose. (D) Cellular MG content (n=4). (E) MG concentration of the culture <t>medium</t> (n=4). (F) MG-H1 content of cell protein. (G) Flux of release of MG-H1 free adduct into the culture medium. (H) FL content of cell protein. (I) Flux of release of FL free adduct into the culture medium. Data are mean±SD (C) n=3; (D–G) n=4; (H and I) n=5). *P<0.05; **p<0.01; ***p<0.001; Student’s t-test . FL, N ε -fructosyl-lysine; HG, high glucose; LG, low glucose.
Alpha Modified Eagle’s Minimum Essential Medium, supplied by BioWhittaker Molecular Applications, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Lonza alfa-mem medium
Dicarbonyl stress in human periodontal ligament cells in high glucose concentration cultures in vitro. (A) Protein glycation by methylglyoxal (MG) forming arginine-derived MG-H1 adduct. (B) Metabolism of MG by the glyoxalase system. (C–I) Culture of periodontal ligament <t>fibroblasts</t> <t>(PDLFs)</t> for 3 days and effect of glucose concentration. (C) Viable cell number and effect of initial glucose concentration. Key: x─ ─ x, 5 mM glucose; ○─○, 8 mM glucose and ●─●, 25 mM glucose. (D) Cellular MG content (n=4). (E) MG concentration of the culture <t>medium</t> (n=4). (F) MG-H1 content of cell protein. (G) Flux of release of MG-H1 free adduct into the culture medium. (H) FL content of cell protein. (I) Flux of release of FL free adduct into the culture medium. Data are mean±SD (C) n=3; (D–G) n=4; (H and I) n=5). *P<0.05; **p<0.01; ***p<0.001; Student’s t-test . FL, N ε -fructosyl-lysine; HG, high glucose; LG, low glucose.
Alfa Mem Medium, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Corning Life Sciences α-mem culture medium
Dicarbonyl stress in human periodontal ligament cells in high glucose concentration cultures in vitro. (A) Protein glycation by methylglyoxal (MG) forming arginine-derived MG-H1 adduct. (B) Metabolism of MG by the glyoxalase system. (C–I) Culture of periodontal ligament <t>fibroblasts</t> <t>(PDLFs)</t> for 3 days and effect of glucose concentration. (C) Viable cell number and effect of initial glucose concentration. Key: x─ ─ x, 5 mM glucose; ○─○, 8 mM glucose and ●─●, 25 mM glucose. (D) Cellular MG content (n=4). (E) MG concentration of the culture <t>medium</t> (n=4). (F) MG-H1 content of cell protein. (G) Flux of release of MG-H1 free adduct into the culture medium. (H) FL content of cell protein. (I) Flux of release of FL free adduct into the culture medium. Data are mean±SD (C) n=3; (D–G) n=4; (H and I) n=5). *P<0.05; **p<0.01; ***p<0.001; Student’s t-test . FL, N ε -fructosyl-lysine; HG, high glucose; LG, low glucose.
α Mem Culture Medium, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Corning Life Sciences α-modified eagle's medium
Dicarbonyl stress in human periodontal ligament cells in high glucose concentration cultures in vitro. (A) Protein glycation by methylglyoxal (MG) forming arginine-derived MG-H1 adduct. (B) Metabolism of MG by the glyoxalase system. (C–I) Culture of periodontal ligament <t>fibroblasts</t> <t>(PDLFs)</t> for 3 days and effect of glucose concentration. (C) Viable cell number and effect of initial glucose concentration. Key: x─ ─ x, 5 mM glucose; ○─○, 8 mM glucose and ●─●, 25 mM glucose. (D) Cellular MG content (n=4). (E) MG concentration of the culture <t>medium</t> (n=4). (F) MG-H1 content of cell protein. (G) Flux of release of MG-H1 free adduct into the culture medium. (H) FL content of cell protein. (I) Flux of release of FL free adduct into the culture medium. Data are mean±SD (C) n=3; (D–G) n=4; (H and I) n=5). *P<0.05; **p<0.01; ***p<0.001; Student’s t-test . FL, N ε -fructosyl-lysine; HG, high glucose; LG, low glucose.
α Modified Eagle's Medium, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
FUJIFILM minimum essential medium α (memα
Dicarbonyl stress in human periodontal ligament cells in high glucose concentration cultures in vitro. (A) Protein glycation by methylglyoxal (MG) forming arginine-derived MG-H1 adduct. (B) Metabolism of MG by the glyoxalase system. (C–I) Culture of periodontal ligament <t>fibroblasts</t> <t>(PDLFs)</t> for 3 days and effect of glucose concentration. (C) Viable cell number and effect of initial glucose concentration. Key: x─ ─ x, 5 mM glucose; ○─○, 8 mM glucose and ●─●, 25 mM glucose. (D) Cellular MG content (n=4). (E) MG concentration of the culture <t>medium</t> (n=4). (F) MG-H1 content of cell protein. (G) Flux of release of MG-H1 free adduct into the culture medium. (H) FL content of cell protein. (I) Flux of release of FL free adduct into the culture medium. Data are mean±SD (C) n=3; (D–G) n=4; (H and I) n=5). *P<0.05; **p<0.01; ***p<0.001; Student’s t-test . FL, N ε -fructosyl-lysine; HG, high glucose; LG, low glucose.
Minimum Essential Medium α (Memα, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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minimum essential medium α (memα - by Bioz Stars, 2026-06
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PAN - Biotech alpha mem eagle medium
Dicarbonyl stress in human periodontal ligament cells in high glucose concentration cultures in vitro. (A) Protein glycation by methylglyoxal (MG) forming arginine-derived MG-H1 adduct. (B) Metabolism of MG by the glyoxalase system. (C–I) Culture of periodontal ligament <t>fibroblasts</t> <t>(PDLFs)</t> for 3 days and effect of glucose concentration. (C) Viable cell number and effect of initial glucose concentration. Key: x─ ─ x, 5 mM glucose; ○─○, 8 mM glucose and ●─●, 25 mM glucose. (D) Cellular MG content (n=4). (E) MG concentration of the culture <t>medium</t> (n=4). (F) MG-H1 content of cell protein. (G) Flux of release of MG-H1 free adduct into the culture medium. (H) FL content of cell protein. (I) Flux of release of FL free adduct into the culture medium. Data are mean±SD (C) n=3; (D–G) n=4; (H and I) n=5). *P<0.05; **p<0.01; ***p<0.001; Student’s t-test . FL, N ε -fructosyl-lysine; HG, high glucose; LG, low glucose.
Alpha Mem Eagle Medium, supplied by PAN - Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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STEMCELL Technologies Inc alpha-mem medium
Dicarbonyl stress in human periodontal ligament cells in high glucose concentration cultures in vitro. (A) Protein glycation by methylglyoxal (MG) forming arginine-derived MG-H1 adduct. (B) Metabolism of MG by the glyoxalase system. (C–I) Culture of periodontal ligament <t>fibroblasts</t> <t>(PDLFs)</t> for 3 days and effect of glucose concentration. (C) Viable cell number and effect of initial glucose concentration. Key: x─ ─ x, 5 mM glucose; ○─○, 8 mM glucose and ●─●, 25 mM glucose. (D) Cellular MG content (n=4). (E) MG concentration of the culture <t>medium</t> (n=4). (F) MG-H1 content of cell protein. (G) Flux of release of MG-H1 free adduct into the culture medium. (H) FL content of cell protein. (I) Flux of release of FL free adduct into the culture medium. Data are mean±SD (C) n=3; (D–G) n=4; (H and I) n=5). *P<0.05; **p<0.01; ***p<0.001; Student’s t-test . FL, N ε -fructosyl-lysine; HG, high glucose; LG, low glucose.
Alpha Mem Medium, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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STEMCELL Technologies Inc α-mem semi-solid matrix culture medium
Dicarbonyl stress in human periodontal ligament cells in high glucose concentration cultures in vitro. (A) Protein glycation by methylglyoxal (MG) forming arginine-derived MG-H1 adduct. (B) Metabolism of MG by the glyoxalase system. (C–I) Culture of periodontal ligament <t>fibroblasts</t> <t>(PDLFs)</t> for 3 days and effect of glucose concentration. (C) Viable cell number and effect of initial glucose concentration. Key: x─ ─ x, 5 mM glucose; ○─○, 8 mM glucose and ●─●, 25 mM glucose. (D) Cellular MG content (n=4). (E) MG concentration of the culture <t>medium</t> (n=4). (F) MG-H1 content of cell protein. (G) Flux of release of MG-H1 free adduct into the culture medium. (H) FL content of cell protein. (I) Flux of release of FL free adduct into the culture medium. Data are mean±SD (C) n=3; (D–G) n=4; (H and I) n=5). *P<0.05; **p<0.01; ***p<0.001; Student’s t-test . FL, N ε -fructosyl-lysine; HG, high glucose; LG, low glucose.
α Mem Semi Solid Matrix Culture Medium, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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StemCells Inc culture medium α-mem human pdl
Dicarbonyl stress in human periodontal ligament cells in high glucose concentration cultures in vitro. (A) Protein glycation by methylglyoxal (MG) forming arginine-derived MG-H1 adduct. (B) Metabolism of MG by the glyoxalase system. (C–I) Culture of periodontal ligament <t>fibroblasts</t> <t>(PDLFs)</t> for 3 days and effect of glucose concentration. (C) Viable cell number and effect of initial glucose concentration. Key: x─ ─ x, 5 mM glucose; ○─○, 8 mM glucose and ●─●, 25 mM glucose. (D) Cellular MG content (n=4). (E) MG concentration of the culture <t>medium</t> (n=4). (F) MG-H1 content of cell protein. (G) Flux of release of MG-H1 free adduct into the culture medium. (H) FL content of cell protein. (I) Flux of release of FL free adduct into the culture medium. Data are mean±SD (C) n=3; (D–G) n=4; (H and I) n=5). *P<0.05; **p<0.01; ***p<0.001; Student’s t-test . FL, N ε -fructosyl-lysine; HG, high glucose; LG, low glucose.
Culture Medium α Mem Human Pdl, supplied by StemCells Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Dicarbonyl stress in human periodontal ligament cells in high glucose concentration cultures in vitro. (A) Protein glycation by methylglyoxal (MG) forming arginine-derived MG-H1 adduct. (B) Metabolism of MG by the glyoxalase system. (C–I) Culture of periodontal ligament fibroblasts (PDLFs) for 3 days and effect of glucose concentration. (C) Viable cell number and effect of initial glucose concentration. Key: x─ ─ x, 5 mM glucose; ○─○, 8 mM glucose and ●─●, 25 mM glucose. (D) Cellular MG content (n=4). (E) MG concentration of the culture medium (n=4). (F) MG-H1 content of cell protein. (G) Flux of release of MG-H1 free adduct into the culture medium. (H) FL content of cell protein. (I) Flux of release of FL free adduct into the culture medium. Data are mean±SD (C) n=3; (D–G) n=4; (H and I) n=5). *P<0.05; **p<0.01; ***p<0.001; Student’s t-test . FL, N ε -fructosyl-lysine; HG, high glucose; LG, low glucose.

Journal: BMJ Open Diabetes Research & Care

Article Title: Glycolytic overload-driven dysfunction of periodontal ligament fibroblasts in high glucose concentration, corrected by glyoxalase 1 inducer

doi: 10.1136/bmjdrc-2020-001458

Figure Lengend Snippet: Dicarbonyl stress in human periodontal ligament cells in high glucose concentration cultures in vitro. (A) Protein glycation by methylglyoxal (MG) forming arginine-derived MG-H1 adduct. (B) Metabolism of MG by the glyoxalase system. (C–I) Culture of periodontal ligament fibroblasts (PDLFs) for 3 days and effect of glucose concentration. (C) Viable cell number and effect of initial glucose concentration. Key: x─ ─ x, 5 mM glucose; ○─○, 8 mM glucose and ●─●, 25 mM glucose. (D) Cellular MG content (n=4). (E) MG concentration of the culture medium (n=4). (F) MG-H1 content of cell protein. (G) Flux of release of MG-H1 free adduct into the culture medium. (H) FL content of cell protein. (I) Flux of release of FL free adduct into the culture medium. Data are mean±SD (C) n=3; (D–G) n=4; (H and I) n=5). *P<0.05; **p<0.01; ***p<0.001; Student’s t-test . FL, N ε -fructosyl-lysine; HG, high glucose; LG, low glucose.

Article Snippet: Primary human PDLFs (cat. no. 2630) and Modified Eagles Medium (MEM) (cat. no. M8042) were purchased from ScienCell (Carlsbad, USA).

Techniques: Concentration Assay, In Vitro, Derivative Assay